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single cell capture  (10X Genomics)

 
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    Structured Review

    10X Genomics single cell capture
    Design of individual barcoded lentiviral vectors Schematic of a lentiviral vector encoding a transcription factor (TF) and corresponding 20-bp barcode sequence. An example of unique 8-bp barcode (orange) flanked by 6-bp constant regions (gray) is shown. Viral elements are indicated in gray boxes, including 5′ and 3′ long terminal repeats (LTR), the constitutive splenic focus-forming virus (SFFV) promoter, the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and the poly(A) sequence. The barcode is optimally positioned approximately 300 bp upstream of the poly(A) tail to enable detection by <t>3′</t> <t>single-cell</t> mRNA sequencing.
    Single Cell Capture, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single cell capture/product/10X Genomics
    Average 86 stars, based on 1 article reviews
    single cell capture - by Bioz Stars, 2026-05
    86/100 stars

    Images

    1) Product Images from "Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening"

    Article Title: Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2026.104527

    Design of individual barcoded lentiviral vectors Schematic of a lentiviral vector encoding a transcription factor (TF) and corresponding 20-bp barcode sequence. An example of unique 8-bp barcode (orange) flanked by 6-bp constant regions (gray) is shown. Viral elements are indicated in gray boxes, including 5′ and 3′ long terminal repeats (LTR), the constitutive splenic focus-forming virus (SFFV) promoter, the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and the poly(A) sequence. The barcode is optimally positioned approximately 300 bp upstream of the poly(A) tail to enable detection by 3′ single-cell mRNA sequencing.
    Figure Legend Snippet: Design of individual barcoded lentiviral vectors Schematic of a lentiviral vector encoding a transcription factor (TF) and corresponding 20-bp barcode sequence. An example of unique 8-bp barcode (orange) flanked by 6-bp constant regions (gray) is shown. Viral elements are indicated in gray boxes, including 5′ and 3′ long terminal repeats (LTR), the constitutive splenic focus-forming virus (SFFV) promoter, the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and the poly(A) sequence. The barcode is optimally positioned approximately 300 bp upstream of the poly(A) tail to enable detection by 3′ single-cell mRNA sequencing.

    Techniques Used: Plasmid Preparation, Sequencing, Virus, Single Cell

    Representative flow cytometry plots for gating strategy Flow cytometry quantification of cell populations FACS-sorted for single-cell RNA sequencing at reprogramming day 9. The CD45 gate was defined using a fluorescence minus one (FMO) control. Purity of the reprogrammed CD45 + and non-reprogrammed, double-negative (DN; CD45 - HLA-DR - ) populations was assessed. The percentages of cells in each gate are indicated.
    Figure Legend Snippet: Representative flow cytometry plots for gating strategy Flow cytometry quantification of cell populations FACS-sorted for single-cell RNA sequencing at reprogramming day 9. The CD45 gate was defined using a fluorescence minus one (FMO) control. Purity of the reprogrammed CD45 + and non-reprogrammed, double-negative (DN; CD45 - HLA-DR - ) populations was assessed. The percentages of cells in each gate are indicated.

    Techniques Used: Flow Cytometry, Single Cell, RNA Sequencing, Fluorescence, Control



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    Image Search Results


    Design of individual barcoded lentiviral vectors Schematic of a lentiviral vector encoding a transcription factor (TF) and corresponding 20-bp barcode sequence. An example of unique 8-bp barcode (orange) flanked by 6-bp constant regions (gray) is shown. Viral elements are indicated in gray boxes, including 5′ and 3′ long terminal repeats (LTR), the constitutive splenic focus-forming virus (SFFV) promoter, the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and the poly(A) sequence. The barcode is optimally positioned approximately 300 bp upstream of the poly(A) tail to enable detection by 3′ single-cell mRNA sequencing.

    Journal: STAR Protocols

    Article Title: Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening

    doi: 10.1016/j.xpro.2026.104527

    Figure Lengend Snippet: Design of individual barcoded lentiviral vectors Schematic of a lentiviral vector encoding a transcription factor (TF) and corresponding 20-bp barcode sequence. An example of unique 8-bp barcode (orange) flanked by 6-bp constant regions (gray) is shown. Viral elements are indicated in gray boxes, including 5′ and 3′ long terminal repeats (LTR), the constitutive splenic focus-forming virus (SFFV) promoter, the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and the poly(A) sequence. The barcode is optimally positioned approximately 300 bp upstream of the poly(A) tail to enable detection by 3′ single-cell mRNA sequencing.

    Article Snippet: Purification of reprogrammed and non-reprogrammed cell populations , Single-cell capture and library preparation using BD Rhapsody versus 10X Genomics platforms.

    Techniques: Plasmid Preparation, Sequencing, Virus, Single Cell

    Representative flow cytometry plots for gating strategy Flow cytometry quantification of cell populations FACS-sorted for single-cell RNA sequencing at reprogramming day 9. The CD45 gate was defined using a fluorescence minus one (FMO) control. Purity of the reprogrammed CD45 + and non-reprogrammed, double-negative (DN; CD45 - HLA-DR - ) populations was assessed. The percentages of cells in each gate are indicated.

    Journal: STAR Protocols

    Article Title: Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening

    doi: 10.1016/j.xpro.2026.104527

    Figure Lengend Snippet: Representative flow cytometry plots for gating strategy Flow cytometry quantification of cell populations FACS-sorted for single-cell RNA sequencing at reprogramming day 9. The CD45 gate was defined using a fluorescence minus one (FMO) control. Purity of the reprogrammed CD45 + and non-reprogrammed, double-negative (DN; CD45 - HLA-DR - ) populations was assessed. The percentages of cells in each gate are indicated.

    Article Snippet: Purification of reprogrammed and non-reprogrammed cell populations , Single-cell capture and library preparation using BD Rhapsody versus 10X Genomics platforms.

    Techniques: Flow Cytometry, Single Cell, RNA Sequencing, Fluorescence, Control

    Journal: Cell reports

    Article Title: Temporal dynamics of immune cell transcriptomics in brain metastasis progression influenced by gut microbiome dysbiosis

    doi: 10.1016/j.celrep.2025.115356

    Figure Lengend Snippet:

    Article Snippet: ND GBCF prepared cells for 10X Genomics Chromium single cell capture and cDNA libraries according to the standard CITE-seq ( https://citeseq.files.wordpress.com/2019/02/cite-seq_and_hashing_protocol_190213.pdf ) and 10X Genomics standard protocols.

    Techniques: In Vivo, Recombinant, Blocking Assay, Red Blood Cell Lysis, Multiplex Assay, Sequencing, Software, Amplification, Fluorescence